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Retatrutide Protocol

Updated 2mo ago 4 min read 234+ helpful Calculator
Beginner setup

Vial sizes vary by vendor. Smaller bottles are usually 3 mL; larger bottles are often 10 mL. Match the label on the vial you actually bought, then use the calculator below if you need to adjust.

Vial strength 10 mg
Bottle format often 3 mL
BAC water 2 mL
Concentration 5.00 mg/mL

Overview

Retatrutide is a triple agonist peptide targeting GLP-1, GIP, and glucagon receptors, making it one of the most potent metabolic peptides available.

PubMed evidence

  • PMID 37366315 (2023) — Triple-Hormone-Receptor Agonist Retatrutide for Obesity - A Phase 2 Trial. Human trial evidence; supports clinical context without changing the protocol.

  • PMID 37385280 (2023) — Retatrutide, a GIP, GLP-1 and glucagon receptor agonist, for people with type 2 diabetes: a randomised, double-blind, placebo and active-controlled, parallel-group, phase 2 trial conducted in the USA. Human trial evidence; supports clinical context without changing the protocol.

  • PMID 40563436 (2025) — Retatrutide-A Game Changer in Obesity Pharmacotherapy. Review evidence; use as context, not primary protocol input.

Preparation

Recon: 10 mg vial + 2 mL BAC (5 mg/mL).

Protocol

PeriodDoseUnits (@ 5mg/mL)Frequency
Weeks 1-42.5 mg50 u1x/week
Weeks 5-85 mg100 u1x/week
Weeks 9-127.5 mg150 u1x/week
Weeks 13-1610 mg200 u1x/week
Weeks 17-2012.5 mg250 u1x/week
Weeks 21-2415 mg300 u1x/week

Cycle

12 weeks on, 4 weeks off. Half-life is approximately 6 days.

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Dosing Calculator

10 mg + 3 mL BAC · suggested 2 mg
10 mg vial · 3 mL BAC
102030405060708090100
600.60 mL
Concentration3.33 mg/mL
Selected dose2 mg = 60 units
1 unit = 0.01 mL on a U-100 syringe. Doses round to the nearest unit.