Beginner setup
Vial sizes vary by vendor. Smaller bottles are usually 3 mL; larger bottles are often 10 mL. Match the label on the vial you actually bought, then use the calculator below if you need to adjust.
Vial strength 10 mg
Bottle format often 3 mL
BAC water 3 mL
Concentration 3.33 mg/mL
Overview
PE-22-28 (spadin) is a peptide fragment that blocks TREK-1 potassium channels — the same target as a class of experimental antidepressants. It’s a novel mood-and-cognition peptide with a distinctly different mechanism from the Semax family.
Preparation
Reconstitute a 10 mg vial with 3 mL of bacteriostatic water (BAC) for a ~3.33 mg/mL concentration. Refrigerate after mixing.
| Vial | BAC | Concentration |
|---|---|---|
| 10 mg | 3 mL | 3.33 mg/mL |
Protocol
| Period | Dose | Units (@ 3.33 mg/mL) | Frequency |
|---|---|---|---|
| Weeks 1–2 | 50 mcg | 1.5 units (0.015 mL) | 1x/day |
| Weeks 3–8 | 100 mcg | 3 units (0.03 mL) | 1x/day |
| Weeks 9–12 | 150 mcg | 4.5 units (0.045 mL) | 1x/day |
| Weeks 13–16 | 200 mcg | 6 units (0.06 mL) | 1x/day |
Cycle
8–16 week cycles. Doses are tiny — a 0.3 mL (30 unit) syringe is easier to read than a full 1 mL syringe.