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Sleep / Circadian

DSIP Protocol

Updated 5mo ago 4 min read 267+ helpful Calculator
Beginner setup

Vial sizes vary by vendor. Smaller bottles are usually 3 mL; larger bottles are often 10 mL. Match the label on the vial you actually bought, then use the calculator below if you need to adjust.

Vial strength 10 mg
Bottle format often 3 mL
BAC water 3 mL
Concentration 3.33 mg/mL

Overview

DSIP (delta sleep-inducing peptide) is linked to slow-wave sleep and recovery. It’s not a sedative — the research angle is supporting deep, restorative sleep architecture rather than knocking you out.

Preparation

Reconstitute a 10 mg vial with 3 mL of bacteriostatic water (BAC) for a ~3.33 mg/mL concentration. Refrigerate after mixing; aliquot if you plan to keep it long-term.

VialBACConcentration
10 mg3 mL3.33 mg/mL

Protocol

PeriodDoseUnits (@ 3.33 mg/mL)Frequency
Week 1100 mcg3 units (0.03 mL)1x/day
Week 2150 mcg5 units (0.05 mL)1x/day
Week 3200 mcg6 units (0.06 mL)1x/day
Weeks 4–8250–300 mcg8–9 units (0.08–0.09 mL)1x/day

Cycle

4–8 week cycles. Inject 30–60 minutes before bedtime. Small volumes — a 30 or 50 unit syringe makes the low doses readable.

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Dosing Calculator

10 mg + 3 mL · suggested 100 mcg
10 mg vial · 3 mL BAC
102030405060708090100
30.03 mL
Concentration3.33 mg/mL
Selected dose0.1 mg (100 mcg) = 3 units
1 unit = 0.01 mL on a U-100 syringe. Doses round to the nearest unit.