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SS-31 (Elamipretide) Protocol

Updated 3mo ago 4 min read 248+ helpful Calculator
Beginner setup

Vial sizes vary by vendor. Smaller bottles are usually 3 mL; larger bottles are often 10 mL. Match the label on the vial you actually bought, then use the calculator below if you need to adjust.

Vial strength 10 mg
Bottle format often 3 mL
BAC water 1 mL
Concentration 10 mg/mL

Overview

SS-31 (elamipretide) binds cardiolipin on the inner mitochondrial membrane to improve energy production efficiency. It’s one of the most-studied mitochondrial peptides in clinical research.

Preparation

Recon: 10 mg vial + 1 mL BAC (10 mg/mL).

Protocol

PeriodDoseUnits (@ 10 mg/mL)Frequency
Weeks 1–25 mg (5,000 mcg)50 units (0.50 mL)1x/day
Weeks 3–810 mg (10,000 mcg)100 units (1.00 mL)1x/day

Cycle

8–12 week cycles. Start at 5 mg for the first two weeks, then hold at 10 mg — the dose used in most clinical protocols. Advanced runs go to 15–20 mg split across two injections.

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Dosing Calculator

10 mg + 3 mL BAC · suggested 5 mg
10 mg vial · 3 mL BAC
102030405060708090100
1001.00 mL
Concentration3.33 mg/mL
Selected dose5 mg = 150 units · split 2 × 75 u
1 unit = 0.01 mL on a U-100 syringe. Doses round to the nearest unit.