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AICAR Protocol

Updated 2w ago 4 min read 287+ helpful Calculator
Beginner setup

Vial sizes vary by vendor. Smaller bottles are usually 3 mL; larger bottles are often 10 mL. Match the label on the vial you actually bought, then use the calculator below if you need to adjust.

Vial strength 50 mg
Bottle format often 3 mL
BAC water 3 mL
Concentration 17 mg/mL

Overview

AICAR (5-aminoimidazole-4-carboxamide ribonucleoside) activates AMPK, the master energy sensor in cells. It earned the nickname “exercise pill” after sedentary animals given AICAR showed dramatic endurance gains. It’s a WADA-prohibited metabolic modulator, strictly a research compound.

Preparation

Reconstitute a 50 mg vial with 3 mL of bacteriostatic water (BAC) for a ~16.7 mg/mL concentration. Inject slowly down the vial wall and swirl gently — never shake.

VialBACConcentration
50 mg3 mL16.67 mg/mL

Protocol

PeriodDoseUnits (@ 16.67 mg/mL)Frequency
Weeks 1–21,000 mcg (1 mg)6 units (0.06 mL)1x/day
Weeks 3–42,000 mcg (2 mg)12 units (0.12 mL)1x/day
Weeks 5–83,000 mcg (3 mg)18 units (0.18 mL)1x/day

Cycle

8–12 week cycles. Start at 1 mg daily and add 1 mg every 2 weeks as tolerated. Advanced protocols go up to 5 mg daily. WADA-prohibited — not for athletes.

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Dosing Calculator

50 mg + 3 mL · suggested 1 mg
50 mg vial · 3 mL BAC
102030405060708090100
60.06 mL
Concentration16.67 mg/mL
Selected dose1 mg = 6 units
1 unit = 0.01 mL on a U-100 syringe. Doses round to the nearest unit.