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Longevity / Anti-Aging

5-Amino-1MQ Protocol

Updated 4mo ago 4 min read 231+ helpful Calculator

Overview

5-Amino-1MQ activates NMNAT2, the enzyme that recycles NMN back into NAD+. Instead of flooding the system with NAD+ precursors, it works on the recycling side of the equation — a different angle on the same energy pathway.

Preparation

Reconstitute a 50 mg vial with 4 mL of bacteriostatic water (BAC) for a 12.5 mg/mL concentration. Swirl gently; refrigerate after mixing.

VialBACConcentration
50 mg4 mL12.5 mg/mL

Protocol

PeriodDoseUnits (@ 12.5 mg/mL)Frequency
Days 1–22.5 mg20 units (0.20 mL)1x/day
Day 3+5 mg40 units (0.40 mL)1x/day

Cycle

8–12 week cycles. Steady daily dosing builds NMNAT2 activity; no titration past 5 mg in the available research.

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Dosing Calculator

50 mg + 4 mL · suggested 2.5 mg
50 mg vial · 4 mL BAC
102030405060708090100
200.20 mL
Concentration12.50 mg/mL
Selected dose2.5 mg = 20 units
1 unit = 0.01 mL on a U-100 syringe. Doses round to the nearest unit.